Tutorial

This section will present the essential features of Pylake with example code to get you started quickly. Most of the tutorial pages are also available for download as Jupyter notebooks.

Code snippets are included directly within the tutorial text to illustrate features, thus they omit some common and repetitive code (like import statements) in order to save space and avoid distractions. It is assumed that the following lines precede any other code:

import numpy as np
import matplotlib.pyplot as plt

import lumicks.pylake as lk

This uses the common scientific package aliases: np and plt. These import conventions are used consistently in the tutorial.

Contents

• 1. Files and Channels
• 2. FD Curves
• 3. Confocal Scans
• 4. Kymographs
• 5. Image Stacks
• 6. Plotting Images
• 7. Fd Fitting
• 8. Notebook Widgets
• 9. Kymotracking
• 10. Force Calibration
• 11. Population Dynamics
• 12. Piezo Tracking