Pylake 1.4.0¶
Pylake v1.4.0
has been released with new features and improvements to existing analyses. Here’s some of the highlights:
Automatic bead cropping¶
Added estimate_bead_edges()
and crop_beads()
for quickly crop the beads out of a kymograph using an estimate of the bead edges. This can help when batch processing of kymographs.
Filter customization kymotracking¶
We added the option to customize the filters applied prior to peak detection to track_greedy()
.
To do this, we added two additional parameters:
filter_width
allows customizing the filter applied prior to detection.adjacency_filter
applies a filter on the detected peaks, removing any fluorescent peaks that do not have a detected peak in an adjacent frame.
This allows using lower thresholds, while keeping false detections in check.
Other changes¶
In addition, this release contains several other bug-fixes and improvements. For a full list of all the changes, please refer to the full changelog.
Happy Pylake-ing!