lumicks.pylake.point_scan.PointScan¶
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class
PointScan
(name, file, start, stop, json)¶ A confocal point scan exported from Bluelake
Parameters: - name : str
point scan name
- file : lumicks.pylake.File
Parent file. Contains the channel data.
- start : int
Start point in the relevant info wave.
- stop : int
End point in the relevant info wave.
- json : dict
Dictionary containing scan-specific metadata.
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__init__
(name, file, start, stop, json)¶ Initialize self. See help(type(self)) for accurate signature.
Methods
__init__
(name, file, start, stop, json)Initialize self. from_dataset
(h5py_dset, file)Construct a confocal class from dataset. plot_blue
(**kwargs)Plot an image of the blue photon channel plot_green
(**kwargs)Plot an image of the green photon channel plot_red
(**kwargs)Plot an image of the red photon channel plot_rgb
(**kwargs)Plot all color channels Attributes
blue_photon_count
blue_power
center_point_um
Returns a dictionary of the x/y/z center coordinates of the scan (w.r.t. green_photon_count
green_power
has_fluorescence
Deprecated since version 0.8.0.
has_force
Deprecated since version 0.8.0.
json
Deprecated since version 0.8.0.
red_photon_count
red_power
sted_power
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classmethod
from_dataset
(h5py_dset, file)¶ Construct a confocal class from dataset.
Parameters: - h5py_dset : h5py.Dataset
The original HDF5 dataset containing confocal scan information
- file : lumicks.pylake.File
The parent file. Used to loop up channel data
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plot_blue
(**kwargs)¶ Plot an image of the blue photon channel
Parameters: - **kwargs
Forwarded to
matplotlib.pyplot.imshow()
.
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plot_green
(**kwargs)¶ Plot an image of the green photon channel
Parameters: - **kwargs
Forwarded to
matplotlib.pyplot.imshow()
.
-
plot_red
(**kwargs)¶ Plot an image of the red photon channel
Parameters: - **kwargs
Forwarded to
matplotlib.pyplot.imshow()
.
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plot_rgb
(**kwargs)¶ Plot all color channels
Parameters: - **kwargs
Forwarded to
~matplotlib.pyplot.plot
.
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center_point_um
¶ Returns a dictionary of the x/y/z center coordinates of the scan (w.r.t. brightfield field of view)
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has_fluorescence
¶ Deprecated since version 0.8.0: By definition, confocal images always have fluorescence data.
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has_force
¶ Deprecated since version 0.8.0: This property is always False and therefore not needed.
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json
¶ Deprecated since version 0.8.0: Access to raw metadata will be removed in a future release. Use accessor properties instead. (see docs)